Monday, March 12, 2012

lQ interaction with E. coli RNA polymerase for antitermination: Regions of E. coli RNA polymerase required for lQ-mediated antitermination in binding and function


Phage ?Q protein is a transcriptional antiterminator that is required for late gene expression from the pR? promoter when ? prophage decides to follow the lytic pathway. ?Q modifies Escherichia coli RNA polymerase (RNAP) at the early elongation stage. Once engaged, ?Q maintains itself as a subunit of the elongation complex throughout the elongation. Studies show that ?Q confers RNAP resistance to both intrinsic and ?-mediated terminators and less pausing. However, little is known about the interaction between ?Q and RNAP. In this study, the largest subun
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